Construction of recombinant lactococcus lactis expressing porcine transmissible gastroenteritis spike glycoprotein and analysis of immunogenicity 基因的重組乳酸乳球菌的構(gòu)建及免疫原性分析
Sds - page analysis revealed that lactococcus lactis nz9800 harbouring pmg36e / nisa restored little ability of nisin production 以lactococcuslactisnz9800為受體菌,研究了表達載體的表達情況。
Using a mouse model , the uk team has also found that a modified bacterium lactococcus lactis can be used to vaccinate against harmful infections 利用小鼠模型,該英國研究小組已經(jīng)發(fā)現(xiàn)了一種基因修飾細菌(乳酸乳球菌)可用于對有害傳染病的免疫接種。
4 . the recombinant plasmid pmg36e / nisa was introduced into lactococcus lactis nz9800 . sds - page analysis revealed that lactococcus lactis nz9800 harbouring pmg36e / nisa restored no ability of nisin production 抽提ecolijm109中的重組質(zhì)粒,轉(zhuǎn)化l . lactisnz9800 ,在含紅霉素的gm17平板上篩選轉(zhuǎn)化子。
This has resulted in the availability of genetically modified l . lactis with prospects for application in food industry . in this studay , genomic dna extracted from lactococcus lactis nizo r5 was used directly as the template for pcr in this paper 乳酸乳球菌是一類長期應(yīng)用于食品發(fā)酵業(yè)的有益微生物,以乳酸菌為受體的表達系統(tǒng)具有安全、表達產(chǎn)物便于分離,使用方便等優(yōu)點。
A dna fragment was amplified from lactococcus lactis nizo r5 genome by means of pcr and cloned into pmg36e plasmid . the recombinant plasmid was transformd into e . colijm 109 and identified by restriction endonucleases analyzing . the recombinant plasmid was introduced into lactococcus lactis nz9800 本實驗提取乳酸乳球菌nizor5的基因組dna為模板,用pcr方法得到乳鏈菌肽前體基因( nisa ) ,克隆到乳酸乳球菌表達載體pmg36e ,構(gòu)建了表達載體pmg36e nisa 。
A approximately 460bp dna fragment was amplified by pcr from lactococcus lactis nizo r5 genome . the primers used in this study comprised the following nucleotide sequences : 5 " - cgcgaattcgatataggtttattgagt - 3 " and 5 " - atgaagcttatccatgtcagaactaa - 3 " . conditions used for the pcr consisted of 30 cycles of 94 ? for0 . 5min , 45 ? for imin and 72 ? for 1 . 5min , plus one additional cycle of 72 for 10min 根據(jù)已發(fā)表的乳鏈菌肽前體基因的dna序列,設(shè)計兩條引物并引入酶切位點。以lactococcuslactisr5基因組dna為模板, pcr反應(yīng)條件: 94變性30s , 45退火60s , 72延伸90s ,反應(yīng)進行30個循環(huán)。成功擴出一條約460bp的dna片段。
百科解釋
Lactococcus lactis is a Gram-positive bacterium used extensively in the production of buttermilk and cheese, but has recently also become famous as the first genetically modified organism to be used alive for the treatment of human disease. L.